| Asanoinfok | Дата: Воскресенье, 21.04.2013, 14:48 | Сообщение # 1 |
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The vectors that are most commonly used in such transformations are plasmids - small, circular pieces of dna that are replicated in pcr and then inserted into the bacterial genome. these plasmids usually contain not only the target gene - e.g. the one coding for gfp - but also some sort of control gene. this can either be a resistance gene of some sort or one coding for an important metabolic enzyme - there are certain strains of microbes that cannot synthesize some necessary substance, e.g. an amino acid - which serves, suitable growth media provided, to distinguish between microbes that have incorporated the plasmid and those that haven't - if this wasn't done, it might well be that the few bacteria that have incorporated the plasmid are quickly overgrown by the other, genotypically normal bacteria, or that the desired gene isn't expressed, at all, as it would then not be necessary to synthesize the ampicillin antifactor...
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